Research to Business
Offer: 670

Do-it-yourself cell culture carrier made of hydrogel

New photolithographic procedure for photodegradable hydrogels producing individual microstructured cell culture carriers for suspension cultures.

The shape and size of the microarrays and microcavities can be individualized by the UV structuring of the photodegradable hydrogel. (Image: Dr. Pavel Levkin / KIT)

Hydrogels are used for cell cultivation due to their mechanical properties, similar to human tissue. The typical jelly-like consistency results from the high water content of the polymer network. Hydrogels offer better individualization opportunities and provide a more natural environment for cells than traditional microtiter plates.

State of the art

So far, hydrogels for cell culture have been manufactured using soft lithographic methods. Here a negative form is prepared which defines the hole sizes and volumes of the later microcavities. The hydrogel is then molded in the reusable master form: The starting materials are poured in, polymerization is initiated and the hydrogel is gelled.

Technology

Scientists at the Institute of Toxicology and Genetics (ITG) at KIT provide a more flexible manufacturing technique with a photolithographic procedure for photodegradable hydrogels. Instead of mold casting, photomasks are used in an exposure process for structuring. The fused silica photomask images planned channels or cavities with a resolution of up to 50 micrometers using a pattern of closed and open areas. A mostly block-shaped hydrogel blank that has been polymerized from commercially available chemicals (e.g. polymethacrylates) and photo catalyst under UV light or with a redox initiator is irradiated again with the photo mask on top. The UV radiation passes through the translucent areas so that the network structures of the hydrogel dissolve locally and liquefy. After rinsing, the customized wells remain for the parallelization of suspension cultures.

Advantages

The developed, low-cost process enables fast and direct structuring of photochemically degradable hydrogels and does not require additional photolabile chemicals for degradation. Any geometries can be manufactured by simply changing the photomasks. The depth of degradation can be controlled by irradiation time and intensity. Compared to existing methods, the process can be used without special knowledge in polymer chemistry.

Options for companies

The hydrogel arrays were tested in cell tests at the institute. Due to their simple handling, they could be sold as hydrogel kits with standard photomasks. KIT is looking for partners for industrial use and research cooperations.

Images close open

Your contact person for this offer

Frauke Helms, Karlsruhe Institute of Technology (KIT)
Innovation Manager, Innovation and Relations Management (IRM)
Phone: +49 721 608-26036

Email: frauke.helms@kit.edu

Add offer to watch list